Synthesis, characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides.
Identifieur interne : 000345 ( Main/Exploration ); précédent : 000344; suivant : 000346Synthesis, characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides.
Auteurs : RBID : pubmed:23792556Abstract
Semiconductor nanoparticles, also known as quantum dots (QDs), are widely used in biomedical imaging studies and pharmaceutical research. Cell-penetrating peptides (CPPs) are a group of small peptides that are able to traverse cell membrane and deliver a variety of cargoes into living cells. CPPs deliver QDs into cells with minimal nonspecific absorption and toxic effect. In this study, water-soluble, monodisperse, carboxyl-functionalized indium phosphide (InP)/zinc sulfide (ZnS) QDs coated with polyethylene glycol lipids (designated QInP) were synthesized for the first time. The physicochemical properties (optical absorption, fluorescence and charging state) and cellular internalization of QInP and CPP/QInP complexes were characterized. CPPs noncovalently interact with QInP in vitro to form stable CPP/QInP complexes, which can then efficiently deliver QInP into human A549 cells. The introduction of 500nM of CPP/QInP complexes and QInP at concentrations of less than 1μM did not reduce cell viability. These results indicate that carboxylated and polyethylene-glycolylated (PEGylated) bifunctionalized QInP are biocompatible nanoparticles with potential for use in biomedical imaging studies and drug delivery applications.
DOI: 10.1016/j.colsurfb.2013.05.038
PubMed: 23792556
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synthesis, characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides.</title><author><name sortKey="Liu, Betty R" uniqKey="Liu B">Betty R Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Natural Resources and Environmental Studies, National Dong Hwa University, 1, Sec. 2, Da-Hsueh Road, Shoufeng, Hualien 97401, Taiwan.</nlm:affiliation><country xml:lang="fr">République de Chine (Taïwan)</country><wicri:regionArea>Department of Natural Resources and Environmental Studies, National Dong Hwa University, 1, Sec. 2, Da-Hsueh Road, Shoufeng, Hualien 97401</wicri:regionArea></affiliation></author><author><name sortKey="Winiarz, Jeffrey G" uniqKey="Winiarz J">Jeffrey G Winiarz</name></author><author><name sortKey="Moon, Jong Sik" uniqKey="Moon J">Jong-Sik Moon</name></author><author><name sortKey="Lo, Shih Yen" uniqKey="Lo S">Shih-Yen Lo</name></author><author><name sortKey="Huang, Yue Wern" uniqKey="Huang Y">Yue-Wern Huang</name></author><author><name sortKey="Aronstam, Robert S" uniqKey="Aronstam R">Robert S Aronstam</name></author><author><name sortKey="Lee, Han Jung" uniqKey="Lee H">Han-Jung Lee</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1016/j.colsurfb.2013.05.038</idno><idno type="RBID">pubmed:23792556</idno><idno type="pmid">23792556</idno><idno type="wicri:Area/Main/Corpus">000564</idno><idno type="wicri:Area/Main/Curation">000564</idno><idno type="wicri:Area/Main/Exploration">000345</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Semiconductor nanoparticles, also known as quantum dots (QDs), are widely used in biomedical imaging studies and pharmaceutical research. Cell-penetrating peptides (CPPs) are a group of small peptides that are able to traverse cell membrane and deliver a variety of cargoes into living cells. CPPs deliver QDs into cells with minimal nonspecific absorption and toxic effect. In this study, water-soluble, monodisperse, carboxyl-functionalized indium phosphide (InP)/zinc sulfide (ZnS) QDs coated with polyethylene glycol lipids (designated QInP) were synthesized for the first time. The physicochemical properties (optical absorption, fluorescence and charging state) and cellular internalization of QInP and CPP/QInP complexes were characterized. CPPs noncovalently interact with QInP in vitro to form stable CPP/QInP complexes, which can then efficiently deliver QInP into human A549 cells. The introduction of 500nM of CPP/QInP complexes and QInP at concentrations of less than 1μM did not reduce cell viability. These results indicate that carboxylated and polyethylene-glycolylated (PEGylated) bifunctionalized QInP are biocompatible nanoparticles with potential for use in biomedical imaging studies and drug delivery applications.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">23792556</PMID><DateCreated><Year>2013</Year><Month>6</Month><Day>24</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4367</ISSN><JournalIssue CitedMedium="Internet"><Volume>111C</Volume><PubDate><Year>2013</Year><Month>Jun</Month><Day>2</Day></PubDate></JournalIssue><Title>Colloids and surfaces. B, Biointerfaces</Title><ISOAbbreviation>Colloids Surf B Biointerfaces</ISOAbbreviation></Journal><ArticleTitle>Synthesis, characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides.</ArticleTitle><Pagination><MedlinePgn>162-170</MedlinePgn></Pagination><ELocationID EIdType="pii">S0927-7765(13)00364-0</ELocationID><ELocationID EIdType="doi">10.1016/j.colsurfb.2013.05.038</ELocationID><Abstract><AbstractText NlmCategory="UNLABELLED">Semiconductor nanoparticles, also known as quantum dots (QDs), are widely used in biomedical imaging studies and pharmaceutical research. Cell-penetrating peptides (CPPs) are a group of small peptides that are able to traverse cell membrane and deliver a variety of cargoes into living cells. CPPs deliver QDs into cells with minimal nonspecific absorption and toxic effect. In this study, water-soluble, monodisperse, carboxyl-functionalized indium phosphide (InP)/zinc sulfide (ZnS) QDs coated with polyethylene glycol lipids (designated QInP) were synthesized for the first time. The physicochemical properties (optical absorption, fluorescence and charging state) and cellular internalization of QInP and CPP/QInP complexes were characterized. CPPs noncovalently interact with QInP in vitro to form stable CPP/QInP complexes, which can then efficiently deliver QInP into human A549 cells. The introduction of 500nM of CPP/QInP complexes and QInP at concentrations of less than 1μM did not reduce cell viability. These results indicate that carboxylated and polyethylene-glycolylated (PEGylated) bifunctionalized QInP are biocompatible nanoparticles with potential for use in biomedical imaging studies and drug delivery applications.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList><Author><LastName>Liu</LastName><ForeName>Betty R</ForeName><Initials>BR</Initials><Affiliation>Department of Natural Resources and Environmental Studies, National Dong Hwa University, 1, Sec. 2, Da-Hsueh Road, Shoufeng, Hualien 97401, Taiwan.</Affiliation></Author><Author><LastName>Winiarz</LastName><ForeName>Jeffrey G</ForeName><Initials>JG</Initials></Author><Author><LastName>Moon</LastName><ForeName>Jong-Sik</ForeName><Initials>JS</Initials></Author><Author><LastName>Lo</LastName><ForeName>Shih-Yen</ForeName><Initials>SY</Initials></Author><Author><LastName>Huang</LastName><ForeName>Yue-Wern</ForeName><Initials>YW</Initials></Author><Author><LastName>Aronstam</LastName><ForeName>Robert S</ForeName><Initials>RS</Initials></Author><Author><LastName>Lee</LastName><ForeName>Han-Jung</ForeName><Initials>HJ</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType>JOURNAL ARTICLE</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>6</Month><Day>2</Day></ArticleDate></Article><MedlineJournalInfo><MedlineTA>Colloids Surf B Biointerfaces</MedlineTA><NlmUniqueID>9315133</NlmUniqueID><ISSNLinking>0927-7765</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>3</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>5</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>5</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0927-7765(13)00364-0</ArticleId><ArticleId IdType="doi">10.1016/j.colsurfb.2013.05.038</ArticleId><ArticleId IdType="pubmed">23792556</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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